The classical variant of the "Isolation of piperine from black pepper" is easily performed. For an extraction of a product from natural sources the yield depends heavily on the quality of the biological raw material and thus cannot be evaluated easily.

Likewise mass efficiency and energy efficiency cannot be easily evaluated because they depend on the yield.

Even though (eco)toxicological data for the educt and the product are not available, acute or chronic toxicological hazards are not to be expected, because the educt has been used as a spice for hundreds of years. For the product piperine ecotoxicological data are not available. The ethanolic potassium hydroxide used in the purification is dangerous because of its corrosive properties, but appropriate safety measures can easily be taken to prevent this hazard. Sufficient (eco)toxicological data are available for all other solvents used. The elution solvent for thin layer chromatography acetic acid ethyl ester has low acute toxicity and no chronic toxicity. In contrast, the solvent dichloromethane is a suspected carcinogen. It can possibly be replaced by another solvent with similar polarity. The acute toxicity of the solvents is relatively low .

All organic substances used in this experiment are biologically easily degradable and mineralizable. Only the solvents have been tested for ecotoxicity so far and exhibit only a low to medium toxicity for aquatic organisms .

Summed up we evaluate this experiment with an average economic efficiency and low toxicological risks, but partly unknown ecotoxicological risks, but a low environmental persistence for most of the substances with the "yellow light".