The classical variant of the "Isolation of hesperidine from orange peel" is easily performed. For an extraction of a product from natural sources the yield depends heavily on the quality of the biological raw material and thus cannot be evaluated easily.

Likewise mass efficiency and energy efficiency cannot be easily evaluated because they depend on the yield.

(Eco)toxicological data for the educt and the product hesperidine are incomplete. No data are available on chronic toxicity. According to theoretical prediction methods no chronic toxicity is suspected. For the solvents used in this experiment relevant (eco)toxicological data are available. Only methanol exhibits a significant acute toxicity. The other organic solvents dimethyl sulfoxide, petroleum ether (40/60), 2-propanol, 1-butanol and acetic acid are of relatively low acute toxicity and do not exhibit chronic toxicity .

Except for petroleum ether (40/60) all organic substances in this experiment are biologically easily degradable and mineralizable. Only the solvents have been evaluated for ecotoxicity and possess a low to medium toxicity for aquatic organisms .

Summed up we evaluate this experiment with an average economic efficiency and low toxicological risks, but partly unknown ecotoxicological risks, but a low environmental persistence for most of the substances with the "yellow light".