| Overview |
| Instructions |
| Operating scheme |
| Substances |
| Equipment |
| Evaluation |
| Analytics |
| - Chromatogram |
| - 1H-NMR |
| - 13C-NMR |
| - IR |
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; Side reactions |
Chromatogram |
| HPLC: crude product | |
| column | Phenomenex Luna C18; particle diameter 3 µm, L=150 mm, ID= 4.6 mm |
| column temperature | 25 °C |
| injection | 5 µL |
| mobile phase | 5% MeCN / H2O, gradient to 95% MeCN / H2O (40 min), 10 min isocratic |
| flow | 1.0 mL/min |
| detector (UV 220 nm) | percent concentration calculated from relative peak area |
| HPLC: pure product | |
| column | Phenomenex Luna C18; particle diameter 3 µm, L=150 mm, ID=4.6 mm |
| column temperature | 25 °C |
| injection | 5 µL |
| mobile phase | 5% MeCN/H2O, gradient to 95% MeCN/H2O (40 min), 10 min isocratic |
| flow | 1.0 mL/min |
| detector (UV 220 nm) | percent concentration calculated from relative peak area |